n-glycosylation site meaning in English
n-糖基化位点
Examples
- The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene , this intron contains donor sequence - gtatgc , lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene . this gene encodes a peptide of 467amino acid residues with molecular weight of 51 . 37kda , containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明， pcr扩增产物中包含有完整的phya基因，该基因全长1506bp ，其中包含一段长102bp的内含子，该内含子具有真菌植酸酶基因内含子的特征保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。该基因编码467个氨基酸，理论分子量为51 . 37kda ，其上有13个潜在的n -糖基化位点， n端19个氨基酸为信号肽序列，植酸酶活性位点序列( crvtfaqvlsrhgaryptdskgk )位于氨基酸序列的+ 71 + 93 。 - Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus , and no transmembrane region was present , suggesting it might be a type of secretory protein . some generic and atipical n - glycosylation sites were present in clsp , suggesting that the protein might represent a glycoprotein . the clsp protein contained one cub ( clr / cls , an embryonic sea urchin protein uegf , and bone morphogenetic protein i ) domain from 39th to 164th ammo acids , which is known to be involved in protein - protein or protein - substrate interaction , and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids
Clsp分子具有补体样丝氨酸蛋白酶的多种结构特征，包括36个氨基酸组成的疏水信号肤，一个cub ( clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl )结构域和一个胰酶样丝氨酸蛋白酶结构域( t汀psin一likeserineproteasedomain )和几个保守的糖基化位点等，没有发现有跨膜区的存在。 - It is divided to extracellular and intracellular part by transmembrane domain . there are 13 n - glycosylation sites , 20 protein kinase c phosphorylation sites , 28 casein kinase ii phosphorylation sites , 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein . an integrin recognition sequences rod lies in intracellular part of bt - r3 protein
跨膜区域( tmd )将它分为胞内和胞外两个部分，它的胞外有13个潜在的糖基化位点， 20个蛋白激酶c的磷酸化位点， 28个酪蛋白激酶的磷酸化位点， 4个酪氨酸酶的磷酸化位点， 15个豆蔻(十四烷基)酰化位点；它的胞内有1个整合蛋白( integrin )识别位点。 - Sequence analysis showed that the full length of this cdna which encodes 364 amino acids is 1398bp . it has 71 % and 69 % identities to lycopersicon esculentum and pisum sativum respectively in amino acid level . this gene is a membrane protein which has one signal peptide , seven transmembrane helices , three n - glycosylation sites and one o - glycosylation site
序列分析表明，该基因的cdna全长为1398bp ，开放阅读框为1095bp ，编码一个364个氨基酸的多肽，与番茄和豌豆中的该家族基因分别具有71和69的氨基酸同源性，是一种膜蛋白，具有1个信号肽序列， 7个跨膜螺旋， 3个n ?糖基化位点和1个o ?糖基化位点，分子量大约为39 . 174kd ，等电点为8 . 07 。 - The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain . in arc1 protein , there are some active sites including n - glycosylation sites , camp - and cgmp - dependent protein kinase phosphorylation sites , protein kinase c phosphorylation sites , casein kinase ii phosphorylation sites , tyrosine kinase phosphorylation sites , n - myristoylation sites , amidation sites and leucine zipper pattern . it probably take part in the signaling process of self - incompatibility
同时在arc1蛋白质中还发现了拉链结构和多个磷酸化位点，包括camp和cgmp依赖的蛋白激酶磷酸化位点、蛋白激酶c磷酸化位点、酪蛋白激酶磷酸化位点、酪氨酸激酶磷酸化位点、糖基化位点等，拉链结构为arc1蛋白之间及与其它蛋白的相互作用提供了可能，而磷酸化位点是arc1参与信号传导过程所必需的。